PCR Supplies
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer both detergent free, 1,000U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes Tween/Triton free 10x Ammonium Buffer and 10x Combination Buffer
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer, Tween free, 5,000U
AS ONE HS Taq DNA Polymerase is a hot start polymerase designed to diminish the formation of non-specific priming events during reaction set up and the first ramp of thermal cycling. HS Taq features high specificity and sensitivity, greater yield compared to standard DNA polymerases. HS Taq enables the detection of low abundance tragets. HS Taq Polymerase includes Tween free 10x Ammonium Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Combination Buffer both detergent free, 500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes Tween/Triton free 10x Ammonium Buffer and 10x Combination Buffer.
INTACT GENOMICS INC T4 UvsX DNA Recombinase, Glycerol Free 500µg
Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange.
INTACT GENOMICS INC Hot Start Taq 2x Master Mix 500 Reactions
Hot start Taq DNA Polymerase 2x master mix is a ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with Intact Genomics 5X Magic Enhancer for efficient amplification of GC rich templates to 84%.Applications Routine PCR and RT-PCR Primer extension Colony PCR Genotyping Efficient for amplifying high GC content template DNA with Magic Enhancer.
Meridian Life Science Air-Dryable Direct RNA/DNA, 5ML
Air-Dryable RNA/DNA qPCR Blood is a 4x qPCR master mix for the development of both DNA and RNA blood tests from whole blood, plasma, and serum samples. The mix has been formulated specifically to overcome the inhibitors found in blood samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or oven dried to create ambient-temperature stable whole blood tests, plasma tests or serum tests.
Meridian Life Science dNTP Mix 40 mM in Lithium salt, 5ML
Ready-to-use Ultra pure sodium salt dNTPs are available in multiple concentrations and supplied in purified water at pH 7.5. dNTPs are enzymatically synthesized from quality raw materials, using highly specific production systems. The manufacturing process eliminates impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates, and pyrophosphates commonly observed in other commercially available dNTP products (>99% purity determined by quantitative HPLC) and are perfectly suited for even the most demanding applications, such as long PCR or highly sensitive qPCR.
INTACT GENOMICS INC Bsu DNA Polymerase Large Fragment, Lyophilized 1000 Units (5 units/µl)
Lyophilized Bsu DNA Polymerase I, Large Fragment is suitable for various purposes including RPA (Recombinase Polymerase Amplification).Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.
Meridian Life Science Bst DNA Polymerase, 20ML
Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesize DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and Loop-Mediated Isothermal DNA Amplification (LAMP). It is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance and sensitivity, speeding up time for optimization. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer is available that is specifically designed for high performance when using crude samples such as sputum or saliva.
Meridian Life Science Air-Dryable Direct RNA/DNA, 1ML
Air-Dryable RNA/DNA qPCR Stool is a 4x qPCR master mix for the development of both DNA and RNA stool tests from crudely processed stool samples. The mix has been formulated specifically to overcome the inhibitors found in stool samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or oven dried to create ambient-temperature stable tests.
Meridian Life Science Lyo-Ready Direct DNA qPCR, 50ML
Lyo-Ready DNA qPCR Stool is a glycerol-free, high-concentration, ready-to-use qPCR formulation engineered with a proprietary buffer system designed for overcoming inhibitors found in stool. It allows accurate target amplification direct from stool samples and can be used in multiplex assays that require minimal sample processing and fast turn-around times without compromising sensitivity, for low copy number samples. It also increase reproducibility in extracted samples, as extraction methods are not always 100% efficient, often resulting in loss of target nucleic acid and potential co-purification of stool PCR inhibitors, such as bile, that can interfere with amplification, leading to false-negative results. It is ideal for room temperature, lyophilized, singleplex and multiplex qPCR assays and is suited for high-throughput, automated platforms.
Meridian Life Science Lyo-Ready Direct DNA qPCR, 1ML
Lyo-Ready DNA qPCR Urine is a glycerol-free, high-concentration, ready-to-use qPCR formulation engineered with a proprietary buffer system designed for overcoming inhibitors found in urine. It allows accurate target amplification direct from urine or pooled urine samples. It can be used in multiplex assays that require minimal sample processing and fast turn-around times without compromising sensitivity, for low copy number samples. It also increase reproducibility in extracted samples, as extraction methods are not always 100% efficient, often resulting in loss of target nucleic acid and potential co-purification of urine PCR inhibitors that can interfere with amplification, leading to false-negative results. It is ideal for room temperature, lyophilized, singleplex and multiplex qPCR assays and is suited for high-throughput, automated platforms.
Meridian Life Science dNTP Set 100mM in Lithium salt, 1ML
Ready-to-use Ultra pure sodium salt dNTPs are available in multiple concentrations and supplied in purified water at pH 7.5. dNTPs are enzymatically synthesized from quality raw materials, using highly specific production systems. The manufacturing process eliminates impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates, and pyrophosphates commonly observed in other commercially available dNTP products (>99% purity determined by quantitative HPLC) and are perfectly suited for even the most demanding applications, such as Long PCR or highly sensitive qPCR.
